Testing Proteins with Biurets Reagent

Testing Proteins with Biurets Reagent Introduction Protein is a major and indispensable word form of cellular macromolecule . There be some proteins that service of process stick out cells and former(a)s that ar enzymes which help increase the tread of cellular reactions. non only do proteins provide vital functions in alive organisms but they are also used to sway substances surrounded by membranes and help regulate activity of other proteins and DNA. They either feed as hormones or help contend against infectious microorganisms. These proteins brood of macromolecules which are polymers consisting of one or to a greater extent chains that are un-branched from monomers that are called amino acids. This molecule consists of both(prenominal) an amino and a carboxyl stem. Proteins can contain from as few as tether to fifty-thousand amino acid units and govern from shape and form, to organism completely soluble or indissoluble to water solutions. When a protein i s formed it is linked together by a peptide sequester using covalent bonds to form surrounded by an amino group of one amino acid and a carboxyl group. likewise dipeptides are formed when the peptide bond joins the two amino acids. Futhermore protiens are also linked by linking three amino acids and this is called a polypeptide.

The purpose of this experiment was to determine protein engrossment in antithetic kind of substances using Biurets Reagent and assess if peptide bonds were present.If Biurets Reagent is added to the substances then(prenominal) the substances lead smorgasbord to the color of Biuret s Reagent. When adding Biurets Reagent to th! e different substances it will pass on in a change of color of blue depending on how many peptide bonds exist. If none exist then there will be no change in the substance. Materials The materials that were used in this experiment were instruments such(prenominal) as: Beakers disposable pipettes, test organ pipes, test tube holders, Chinese markers. The substances that were used were: Distilled H2O, glucose, starch, egg whites, sports drink,...If you want to get a replete(p) essay, order it on our website: BestEssayCheap.com

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